Journal: Scientific Reports

Article Title: All-optical regulation of gene expression in targeted cells

doi: 10.1038/srep05346

Figure Lengend Snippet: (a). TNF-α can be detected with or without ionomycin and Ca 2+ treatment in HeLa cells as an endogenous gene tested by Western Blot. Tubulin: internal control in Western Blot. (b). Immunofluorescence microscopy of TNF-α in HeLa cells. Right: Upregulation of TNF-α in the cells with laser treatment (n = 40 cells in each experiment). The expression is more efficient if the laser-exposure duration is longer. Error bar: standard error of the mean. (c). Demonstration of NFAT-linked luciferase reporter plasmid that was transfected into HeLa cells. (d). Immunofluorescence microscopy of luciferase in HeLa cells. Around 14% cells were transfected with NFAT-luciferase plasmid. If luciferase was expressed, there would be bright fluorescence in the cells indicated by yellow arrows. Right: The expression efficiency of luciferase by the optical method is close to the transfection efficiency determined by ionomycin and Ca 2+ -injection treatment. Error bar: standard error of the mean. Bar: 50 μm.

Article Snippet: The antibody Anti-Human TNF-α (Rabbit) antibody (Rockland, PA) was used for Western Blot.

Techniques: Western Blot, Control, Immunofluorescence, Microscopy, Expressing, Luciferase, Plasmid Preparation, Transfection, Fluorescence, Injection

Journal: Scientific Reports

Article Title: All-optical regulation of gene expression in targeted cells

doi: 10.1038/srep05346

Figure Lengend Snippet: (a). Immunofluorescence microscopy of TNF-α in MSCs. Right: Upregulation ratio of TNF-α in MSCs with laser treatment. (b). Immunofluorescence microscopy of Osx in MSCs. The intense fluorescence in nucleus indicates the expression of Osx since Osx accumulates in nucleus as a transcription factor. Right: Significant upregulation of Osx in laser-treated cells. (c). Immunofluorescence microscopy of Runx2 in MSCs. The intense fluorescence in nucleus indicates the expression of Runx2. Right: Significant upregulation of Runx2 in laser-treated cells. Error bar: standard error of the mean. Bar: 50 μm.

Article Snippet: The antibody Anti-Human TNF-α (Rabbit) antibody (Rockland, PA) was used for Western Blot.

Techniques: Immunofluorescence, Microscopy, Fluorescence, Expressing